α-aiolos antibody #39293 (Active Motif)
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Active Motif
α-aiolos antibody #39293
α Aiolos Antibody #39293, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aiolos+(39293)+antibody/aiolos++39293++antibody/pm39560988-214-42-45
Average 90 stars, based on 1 article reviews
α Aiolos Antibody #39293, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aiolos+(39293)+antibody/aiolos++39293++antibody/pm39560988-214-42-45
Average 90 stars, based on 1 article reviews
α-aiolos antibody #39293 - by Bioz Stars,
2026-09
90/100 stars
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ChIP-sequencing:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Sequencing:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), RNA Sequencing:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), In Vitro:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Generated:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Cell Differentiation:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Protein-Protein interactions:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Quantitative RT-PCR:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Control:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Expressing:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Western Blot:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Binding Assay:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Plasmid Preparation:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Mutagenesis:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Transfection:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Transduction:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Luciferase:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Negative Control:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Gene Expression:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Software:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Comparison:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Over Expression:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Infection:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Flow Cytometry:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Suspension:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Staining:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Labeling:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Enzyme-linked Immunosorbent Assay:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Cell Culture:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Chromatin Immunoprecipitation:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), Incubation:Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity Article Snippet: Membranes were blocked with 2% nonfat dry milk in 1X TBST (10 mM Tris [pH 8], 150 mM NaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos represses CD4 + T cell cytotoxic programming via reciprocal regulation of T FH transcription factors and IL-2 sensitivity. Article Snippet: Lysates were separated by SDSPAGE on 10% Bis-Tris Plus Bolt gels (ThermoFisher) and transferred onto0.45 μmnitrocellulosemembrane.Membraneswere blockedwith 2% nonfat drymilk in 1X TBST (10mMTris [pH 8], 150mMNaCl, 0.05% Tween-20), and detection of indicated proteins was carried out using the following antibodies: Article Title: Aiolos promotes CXCR3 expression on Th1 cells via positive regulation of IFN- γ /STAT1 signaling Article Snippet: The following antibodies were used to detect proteins: α-JAK2 (1:1,000; 3230, Cell Signaling Technology), α–pY-STAT4 (1:1,000; 5267, Cell Signaling Technology), α-STAT4 (1:1,000; 2653S, Cell Signaling Technology), α–pY-STAT1 (1:1,000; 9167S, Cell Signaling Technology), α-STAT1 (1:1,000; sc-417, Santa Cruz Biotechnology), Article Title: Aiolos promotes CXCR3 expression on TH1 cells via positive regulation of IFNγ/STAT1 signaling. Article Snippet: The following 469 antibodies were used to detect proteins: α-JAK2 (1:1,000; #3230, Cell Signaling Technology), α-470 pY-STAT4 (1:1,000; #5267, Cell Signaling Technology), α-STAT4 (1:1,000; #2653S, Cell 471 Signaling Technology), α-pY-STAT1 (1:1,000; #9167S, Cell Signaling Technology), α-STAT1 472 (1:1,000; sc-417, Santa Cruz Biotechnology), |